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发布时间:2013-12-23 11:34:13  

Pm34: a new powdery mildew resistancegene transferred from Aegilops tauschii Coss. to common wheat

Name:SONGLIli Major:Biological Sciences

Powdery mildew is a major fungal disease in wheat growing areas worldwide.

Catalogue
Plant material Methods Results

Discussion

Plant materia
the powdery mildew resistant germplasm line NC97BGTD7 the powdery mildew susceptible cultivar Saluda Chinese Spring nullitetrasomic Nulli5D-tetra5A and ditelosomic Dt5DL lines a single spore culture isolate

method
(1)Cross experiments (2)Microsatellite markers analysis (3)The PRC reactions (4)Data analysis Chi-square(x2)tests for goodness-of-fit MAPMAKER\Exp Version3.0b

NC97BGTD7 (R) ╳ Saluda(S) ↓ shortened NCD7(R) ╳ Saluda(S) ↓ F1 hybrid ╳ F1 hybrid ↓ F2 ╳ F2 ↓ F2:3 ↓ F2:4

(①in the greenhouse ② in the field the F2:3 lines grew in 2002-2003 the F2:4 lines grew in 2003-2004)

result

Greenhouse evaluations

Table 1 Segregation ratios for powdery mildew reaction of F2 derived families from the NCD7 X Saluda cross

F2:3 generation The chi-square test value was in good agreement with the expected (X21:2:1 = 2.47, P = 0.29).

result
? The phenotypic classi Wcation of the 10 F2:3lines

is shown in Table 1.
? The chi-square test value was ingood agreement with the expected 1:2:1 ratio of amonogenic trait (21:2:1= 2.47, P = 0.29). ? The observed pheno-typic classes also Wtted the 1:2:1 ratio (21:2:1= 2.48,P = 0.29) conWrming a single gene controlled resistance

Table 2 Segregation ratios for Microsatellite (SSR) markers among F2 individuals in the NCD7 X Saluda population

a AA homozygous for the NCD7 allele b H heterozygous c BB homozygous for the Saluda allele

The three microsatellite makers were co-dominant and segregated in the expected 1:2:1 ratio (Table 2).
No other marker locus order was within a LOD score of 3.0 from this most likely order.

Discussion
?GISH with an St-genomic probe clearly answered questions about chromosome composition and structure of wheat-Th. intermedium partial amphiploids. ? These results indicate that resistance to WSMV, BYDV, rust, and powdery mildew are not located on St-genome chromosome sbut on J- or J S -genome chromosomes.

?In the present study, not only J-genome chromosomes but also JS –genome chromosomes were identified in TE253 and the resistance to powdery mildew and stripe rust may be located either on Jgenome or on J S -genome chromosomes. ?We are presently developing chromosome addition and substitution lines to identify the chromosome location of the powdery mildew and stripe rust resistance genes.

The end
Thank you


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